Journal: Oncotarget

Article Title: SETD6 regulates NF-κB signaling in urothelial cell survival: Implications for bladder cancer

doi: 10.18632/oncotarget.14750

Figure Lengend Snippet: ( A ) Validation of relative SETD6 expression in different bladder cells was carried out using qPCR. p ≤ 0.05 were considered to be statistically significant (*). The samples were measured in triplicates and the experiment was repeated 3 times. ( B ) cDNA array was performed with bladder cancer tissues ( n = 24) representing different stages and SETD6 expression was analyzed by qPCR. ( C ) Basal level of SETD6 protein was detected using anti-SETD6 antibody by western blotting. ( D ) Immunocytochemistry in different bladder cancer cell lines cells showing cytoplasmic and nuclear SETD6. ( E ) SETD6 protein was detected using anti-SETD6 antibody by western blot in bladder cancer tissues ( n = 9) compared to non-cancerous bladder tissues.

Article Snippet: cDNA array (Origene, TissueScanTM Cancer and Normal Tissue cDNA Arrays) was used to determine the level of SETD6 expression in different bladder tissue samples ( n = 24).

Techniques: Biomarker Discovery, Expressing, Western Blot, Immunocytochemistry

Journal: Plant Physiology

Article Title: A KNOTTED1-LIKE HOMEOBOX Protein Regulates Abscission in Tomato by Modulating the Auxin Pathway ^{1 [OPEN]}

doi: 10.1104/pp.114.253815

Figure Lengend Snippet: Abundance of transcripts of KD1 downstream genes. Total RNA isolated from pedicel AZs of wild-type (cv New Yorker [NY]) and TAPG4::antisense KD1 transgenic (line E) plants 4 h after flower removal (A), and freshly harvested wild-type (cv VF36) and Pts mutant (B) plants were used to determine abundance of transcripts of each gene by qRT-PCR. Abundance of tomato 26S rRNA was used as an internal control. The expression ratio of each gene from microarray analysis is shown above the corresponding qRT-PCR column. Different letters indicate significant differences between NY and line E or between cv VF36 and Pts at each time point (Student’s t test, P < 0.05). Results are the means of three biological replicates ± sd. *, Data representing PIN9 were absent in the microarray.

Article Snippet: Microarray Custom oligonucleotide microarrays were fabricated by NimbleGen Systems, Inc. using photolithography directed by the Maskless Array Synthesizer ( Singh-Gasson et al., 1999 ).

Techniques: Isolation, Transgenic Assay, Mutagenesis, Quantitative RT-PCR, Control, Expressing, Microarray